L929 fibroblast bioassay on the in vitro toxicity of SnCl2, H3PO4, Clearfil SE primer and combinations thereof

Authors

  • Nadine Schlueter Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Switzerland, and Division for Cariology, Department of Operative Dentistry and Periodontology, Center for Dental Medicine, University Medical Center, Albert-Ludwigs-University, Freiburg, Germany
  • Adrian Lussi Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Switzerland
  • Carolina Ganss Department of Conservative and Preventive Dentistry, Dental Clinic, Justus Liebig University, Giessen, Germany
  • Reinhard Gruber Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Switzerland

DOI:

https://doi.org/10.61872/sdj-2016-06-146

PMID:

27377293

Keywords:

L929, MTT, SnCl2, trypan blue, adhesive dentistry

Abstract

Stannous chloride (SnCl(2), 35%) can increase microtensile bond strength between a self-etching adhesive and dental hard tissue either alone or in combination with phosphoric acid (H(3)PO(4), 35%). Whereas cell toxicity of H(3)PO(4) has been sufficiently investigated, little is known about the toxicity of concentrated SnCl(2). The present study determined the in vitro toxicity of SnCl(2), H(3)PO(4), the primer of a self-etching adhesive (Clearfil SE) and combinations thereof at three concentrations (0.01%0.3%) in a L929 fibroblast bioassay. Cell viability was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the integrity of cell membranes was visualised by trypan blue staining (cell necrosis assay). Cell viability was impaired at concentrations between 0.09% and 0.13% for SnCl(2), between 0.06% and 0.09% for H(3)PO(4), and between 0.19% and 0.29% for Clearfil SE primer. Combinations of agents showed additive toxic effects. SnCl(2) showed a slightly lower but comparable in vitro toxicity to H(3)PO(4), which gives a perspective for further in vitro, in situ and clinical studies on this issue, and for the intraoral use of SnCl(2) to increase bond strength between an adhesive system and both enamel and dentine.

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Published

2016-06-20

How to Cite

Schlueter, N., Lussi, A., Ganss, C., & Gruber, R. (2016). L929 fibroblast bioassay on the in vitro toxicity of SnCl2, H3PO4, Clearfil SE primer and combinations thereof. SWISS DENTAL JOURNAL SSO – Science and Clinical Topics, 126(6), 566-572. https://doi.org/10.61872/sdj-2016-06-146

How to Cite

Schlueter, N., Lussi, A., Ganss, C., & Gruber, R. (2016). L929 fibroblast bioassay on the in vitro toxicity of SnCl2, H3PO4, Clearfil SE primer and combinations thereof. SWISS DENTAL JOURNAL SSO – Science and Clinical Topics, 126(6), 566-572. https://doi.org/10.61872/sdj-2016-06-146